P-13: Protective Effect of Black Grape Seed Extract on Sperm Quality in Male Rat Adult That Were Treated with Cyclosporine

Background: Infertility is a problem that now affects many couples that are causing the disintegration of many families and as a psychological crisis, a lot of stress on couples has been entered. Cyclosporine (CsA), a cyclic undecapeptideof fungal origin, is a powerful immunosuppressive agent that has markedly improved graft survival rates in organ transplantation. The drug has adverse side effects such as, hirsutism, and gynecomastia. Black grape extract contain a large amount of phenolic compounds in their skin, pulp and seeds. This study was conducted to investigate the effect of black grape seed extract on sperm quality in male rat adult that were treated with CsA. Materials and Methods: In this study 24 adult male rat, weighing 200 ± 20 gr were used. The animals were divided into three groups (control, cyclosporine, cyclosporine+ black grape seed extracts). Cyclosporine A and black grape seed extract were given by gavage at the dose of (30 and 150) mg/kg/day respectively for 45 days. After 45 days animals were sacrificed by dislocation of cervical vertebra and cauda epididymis were mince in 1ml HTF + 4mg/ml Bovin serom Albumin and putting for 30 minutes in CO2 incubator 37°C, after get out sperm in the medium, different parameter such as sperm viability, motility, sperm count and sperm with DNA fragmentation were measurd. Results: CsA causes both testicular and spermatozoal toxicity by affecting the oxidant/antioxidant balance of testis. This study determined it causes decreaed in sperm viability, motility, sperm count and level of serum testestrone. Conclusion: Finding have been shown that CsA can causes decreased sperm count and live sperm and increase sperm with damaged DNA and immature sperm. While black grape seed extract is improved relative to this situation.